Abiotic Factors Influence on Bacillus subtilis IMV B-7023 Phytase Activity

Bacteria of the Bacillus genus can synthesize specific phytase enzymes. This property is especially important for soil bacteria. It helps to mineralize phytin and phytates provide these bacteria plants (in root zone which they live) with available phosphorus. Our previous studies have demonstrated that subtilis IMV B-7023 strain exhibits a activity use phytate as nutrition source. component «Azogran» complex bacterial preparation crop production. As known, abiotic environmental factors influence In particular, changes significantly under different pH temperatures. Solid particles, including nanosized minerals, also bacteria’s enzymatic activity. The on has not previously been studied, so this was aim our research. Methods. studied by measuring amount phosphate released from sodium during reaction, nanomaterials’ growth — cultivation methods. Results. highest B. observed at 28°C. Also, there no 4—6. However, increased 7 did change increasing buffer system 12. Silicon dioxide in media phosphorus source depended nanomaterial concentration. Thus, 0.05 0.5 g/L silicon medium, 8—18%, 5.0 nanoparticles, inhibition 19% observed. At same time, clay mineral bentonite affect conditions. addition, stimulated all concentrations. So, 1.82—3.34 times upon adding 2.54—5.83 into medium. Since optimal values most are within neutral temperatures 50—55°C, show maximum alkaline lower (28°C) stimulation minerals increases competitiveness Conclusions. Abiotic supernagrowth Optimal physicochemical temperature 28°C 7—12. concentrations 0.05, 0.5, increase effect nanoscale depends their type concentration medium obtained results indicate potential ability effectively assimilate soils, due interaction nanoparticles. expands possibility using agricultural technologies.